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This paper was published in Applied Optics and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/ao/abstract.cfm?uri=ao-48-11-2067. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law. See the OSA Author Copyright Permissions page for further information.

Abstract

High-resolution mosaic imaging with multifocal, multiphoton photon-counting microscopy

High-resolution mosaic imaging is performed for the first time to our knowledge with a multifocal, multiphoton, photon-counting imaging system. We present a novel design consisting of a home-built femtosecond Yb-doped KGdWO4 laser with an optical multiplexer, which is coupled with a commercial Olympus IX-71 microscope frame. Photon counting is performed using single-element detectors and an inexpensive electronic demultiplexer and counters.

Citation:
Eric Chandler, Erich Hoover, Jeff Field, Kraig Sheetz, Wafa Amir, Ramon Carriles, Shi-you Ding, and Jeff Squier, "High-resolution mosaic imaging with multifocal, multiphoton photon-counting microscopy," Appl. Opt. 48, 2067-2077 (2009)

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High-resolution mosaic imaging with multifocal, multiphoton photon-counting microscopy

© 2009 The Optical Society